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bmdm viability  (Dojindo Labs)


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    Dojindo Labs bmdm viability
    Bmdm Viability, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 99/100, based on 59848 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bmdm+viability/pm39828906-79-6-15
    Average 99 stars, based on 59848 article reviews
    bmdm viability - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    CCK-8 Assay:

    Article Title: Restriction of mitochondrial oxidation of glutamine or fatty acids enhances intracellular growth of Mycobacterium abscessus in macrophages
    Article Snippet: .. To check the effects of inhibitors, BMDM viability was assessed using a Cell Counting Kit-8 (Dojindo, Japan) according to the manufacturer’s protocol. ..

    Article Title: Restriction of mitochondrial oxidation of glutamine or fatty acids enhances intracellular growth of Mycobacterium abscessus in macrophages.
    Article Snippet: .. To check the effects of inhibitors, BMDM viability was assessed using a Cell Counting Kit-8 (Dojindo, Japan) according to the manufacturer’s protocol. ..



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    bmdm viability  (Dojindo Labs)
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    bmdm viability  (Thermo Fisher)
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    SasD increases macrophage inflammation and decreases survival. (A and B) RAW264.7 macrophages were infected with WT or sasD A50.1 MRSA for 3 h at an MOI of 10. Macrophages were infected for 1 h in the absence of antibiotics; the medium was then replaced with antibiotic- and serum-free medium with or without gentamicin for 1 h and changed to antibiotic free medium. CFU and transcript graphs are shown without gentamicin conditions. (A) Bacterial burden and percent phagocytosed bacteria in RAW264.7 macrophages. The percent phagocytosed bacteria is calculated by the following equation: ([average CFU with gentamicin]/[average CFU without gentamicin]) · 100. (B) Gene expression in RAW264.7 macrophages infected with WT or sasD A50.1 MRSA for 3 h. (C to F) Bone marrow-derived macrophages (BMDMs) were infected with WT or sasD A50.1 MRSA for 3 h at an MOI of 50 in the absence of antibiotics. <t>C.</t> <t>Viability</t> measured by trypan blue staining of BMDMs 3 h postinfection. (D and E) Representative images (D) and quantification of Western blot analyses (E) of <t>BMDM</t> levels of IL-1β and caspase 1. One to three wells were combined per sample, and protein levels are normalized to beta actin in each sample. Arrows denote which band was used for quantification. (F) Gene expression in BMDM macrophages infected with WT or sasD A50.1 MRSA for 3 h. Statistics were tested by Mann-Whitney test. *, P < 0.05; ***, P < 0.001. N = 4 to 7; combination of several experiments; data graphed as mean ± SEM.
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    bmdms viability  (Beyotime)
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    SasD increases macrophage inflammation and decreases survival. (A and B) RAW264.7 macrophages were infected with WT or sasD A50.1 MRSA for 3 h at an MOI of 10. Macrophages were infected for 1 h in the absence of antibiotics; the medium was then replaced with antibiotic- and serum-free medium with or without gentamicin for 1 h and changed to antibiotic free medium. CFU and transcript graphs are shown without gentamicin conditions. (A) Bacterial burden and percent phagocytosed bacteria in RAW264.7 macrophages. The percent phagocytosed bacteria is calculated by the following equation: ([average CFU with gentamicin]/[average CFU without gentamicin]) · 100. (B) Gene expression in RAW264.7 macrophages infected with WT or sasD A50.1 MRSA for 3 h. (C to F) Bone marrow-derived macrophages (BMDMs) were infected with WT or sasD A50.1 MRSA for 3 h at an MOI of 50 in the absence of antibiotics. <t>C.</t> <t>Viability</t> measured by trypan blue staining of BMDMs 3 h postinfection. (D and E) Representative images (D) and quantification of Western blot analyses (E) of <t>BMDM</t> levels of IL-1β and caspase 1. One to three wells were combined per sample, and protein levels are normalized to beta actin in each sample. Arrows denote which band was used for quantification. (F) Gene expression in BMDM macrophages infected with WT or sasD A50.1 MRSA for 3 h. Statistics were tested by Mann-Whitney test. *, P < 0.05; ***, P < 0.001. N = 4 to 7; combination of several experiments; data graphed as mean ± SEM.
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    SasD increases macrophage inflammation and decreases survival. (A and B) RAW264.7 macrophages were infected with WT or sasD A50.1 MRSA for 3 h at an MOI of 10. Macrophages were infected for 1 h in the absence of antibiotics; the medium was then replaced with antibiotic- and serum-free medium with or without gentamicin for 1 h and changed to antibiotic free medium. CFU and transcript graphs are shown without gentamicin conditions. (A) Bacterial burden and percent phagocytosed bacteria in RAW264.7 macrophages. The percent phagocytosed bacteria is calculated by the following equation: ([average CFU with gentamicin]/[average CFU without gentamicin]) · 100. (B) Gene expression in RAW264.7 macrophages infected with WT or sasD A50.1 MRSA for 3 h. (C to F) Bone marrow-derived macrophages (BMDMs) were infected with WT or sasD A50.1 MRSA for 3 h at an MOI of 50 in the absence of antibiotics. C. Viability measured by trypan blue staining of BMDMs 3 h postinfection. (D and E) Representative images (D) and quantification of Western blot analyses (E) of BMDM levels of IL-1β and caspase 1. One to three wells were combined per sample, and protein levels are normalized to beta actin in each sample. Arrows denote which band was used for quantification. (F) Gene expression in BMDM macrophages infected with WT or sasD A50.1 MRSA for 3 h. Statistics were tested by Mann-Whitney test. *, P < 0.05; ***, P < 0.001. N = 4 to 7; combination of several experiments; data graphed as mean ± SEM.

    Journal: Microbiology Spectrum

    Article Title: Novel Requirement for Staphylococcal Cell Wall-Anchored Protein SasD in Pulmonary Infection

    doi: 10.1128/spectrum.01645-22

    Figure Lengend Snippet: SasD increases macrophage inflammation and decreases survival. (A and B) RAW264.7 macrophages were infected with WT or sasD A50.1 MRSA for 3 h at an MOI of 10. Macrophages were infected for 1 h in the absence of antibiotics; the medium was then replaced with antibiotic- and serum-free medium with or without gentamicin for 1 h and changed to antibiotic free medium. CFU and transcript graphs are shown without gentamicin conditions. (A) Bacterial burden and percent phagocytosed bacteria in RAW264.7 macrophages. The percent phagocytosed bacteria is calculated by the following equation: ([average CFU with gentamicin]/[average CFU without gentamicin]) · 100. (B) Gene expression in RAW264.7 macrophages infected with WT or sasD A50.1 MRSA for 3 h. (C to F) Bone marrow-derived macrophages (BMDMs) were infected with WT or sasD A50.1 MRSA for 3 h at an MOI of 50 in the absence of antibiotics. C. Viability measured by trypan blue staining of BMDMs 3 h postinfection. (D and E) Representative images (D) and quantification of Western blot analyses (E) of BMDM levels of IL-1β and caspase 1. One to three wells were combined per sample, and protein levels are normalized to beta actin in each sample. Arrows denote which band was used for quantification. (F) Gene expression in BMDM macrophages infected with WT or sasD A50.1 MRSA for 3 h. Statistics were tested by Mann-Whitney test. *, P < 0.05; ***, P < 0.001. N = 4 to 7; combination of several experiments; data graphed as mean ± SEM.

    Article Snippet: BMDM viability was determined using trypan blue (Gibco) staining and the Countess 3 automatic cell counter (Invitrogen).

    Techniques: Infection, Bacteria, Gene Expression, Derivative Assay, Staining, Western Blot, MANN-WHITNEY